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Advances in Applied Biotechnology: Proceedings of the 2nd by Tong-Cun Zhang, Motowo Nakajima

By Tong-Cun Zhang, Motowo Nakajima

At the ICAB 2014, researchers from world wide will assemble to debate the newest clinical learn, findings and applied sciences referring to Microbial Genetics and Breeding, Optimization and keep an eye on of organic strategies, organic Separation and organic Purification, and Advances in Biotechnology.
This convention will supply a platform for educational trade at the software of biotechnology among household and foreign universities, examine institutes, company specialists and students. The members will concentrate on the overseas improvement and destiny developments. the development will lay a superb starting place for addressing key technical demanding situations in a variety of parts of utilized biotechnology, offering possibilities to advertise the advance and enlargement of the biotechnology industry.

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Extra resources for Advances in Applied Biotechnology: Proceedings of the 2nd International Conference on Applied Biotechnology (ICAB 2014)-Volume II (Lecture Notes in Electrical Engineering)

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The results showed that the growth rate and the final biomass of recombinant strain containing pET-28a(+)-irrE(W) under stress were significantly higher than the control strain (harboring the empty vector pET-28a(+)). In order to obtain the mutants with higher tolerance to stress, IrrE was further directed evolved by error-prone PCR using pET-28a(+)-irrE(W) as a template in this paper. The cell growth performance and tolerance to stress of the evolved strain were investigated. The sequence comparison between the wild and evolved IrrE, and homologous modeling analysis by SWISS MODEL were further studied.

Novacekii were collected and amplified the 16S rDNA fragments. Results proved that the molecular identification of Microcystis, which is based on the conservative fragment of 16S rDNA, was consistent with the traditional morphology judgement, we confirmed that environmental factors have a huge impact on the distribution of Microcystis and the molecular biology techniques based on 16S rDNA sequencing were reliable for Microcystis identification. Keywords Microcystis novacekii identification Á Distribution Á 16S rDNA Á Molecular D.

4). This is consistent with literature reports that the recombinant protein was 71 kDa [6]. 18 D. Guo et al. Fig. 4 SDS-PAGE analysis of the recombinant cassava β-glucosidase expressed in Pichia pastoris GS115 (GS115/pPICZαA as control); M protein marker; 1 supernatant of GS115/ pPICZαA; 2 supernatant of P. 5 Optimization of Temperature and pH for Hydrolytic Activity of Recombinant Cassava β-Glucosidase The recombinant cassava β-glucosidase was sensitive to temperature and pH values. As shown in Fig.

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